The Rapid Response™ Single Drug Test Cassette (Urine) is a rapid chromatographic immunoassay for the qualitative and simultaneous detection of one of the following drugs in a variety of combinations in human urine. This test is an easy to interpret and user-friendly drug screening solution. The cassette is available in both single and multi-drug testing formats.
- Test Cassettes
- Disposable Droppers
- Product Insert
The Rapid Response™ Single Drug Test Cassette (Urine) is a rapid chromatographic immunoassay for the qualitative and simultaneous detection of one of the following drugs in a variety of combinations in human urine. This test is an easy to interpret and user-friendly drug screening solution. The cassette is available in both single and multi-drug testing formats.
Pipette included with each individually packaged cassette
Control (C) and Test (T) regions marked on cassette
Simple procedure: Collect urine, add drops, wait 5 minutes
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Positive: Only one colored band appears, in the control region (C). No apparent colored band appears in the test region (T).
Negative: Two colored bands appear on the membrane. One band appears in the control region (C) and another band appears in the test region (T).
Invalid: NO line appears in the C – Control region, then the result is Invalid.
Please refer to the product insert for more details.
The Rapid Response™ Single Drug Test Cassette (Urine) is a one-step immunoassay in which chemically labeled drugs (drug-protein conjugates) compete for limited antibody binding sites with drugs which may be present in urine. The test membrane strips are pre-coated with drug-protein conjugates on the test band(s). For each strip, the drug antibody-colloidal gold conjugate pad is placed at one end of the membrane. In the absence of drug in the urine, the solution of the colored antibody-colloidal gold conjugate moves along with the sample solution upward chromatographically by capillary action across the membrane to the immobilized drug-protein conjugate zone on the test band region. The colored antibody-gold conjugate then attaches to the drug-protein conjugates to form visible lines as the antibody complex with the drug conjugate. Therefore, the formation of the visible precipitant in the test zone occurs when the test urine is negative for the drug. When the drug is present in the urine, the drug/metabolite antigen competes with the drug-protein conjugate on the test band region for the limited antibody. When a sufficient concentration of the drug is present, it will fill the limited antibody binding sites. This will prevent attachment of the colored antibody-colloidal gold conjugate to the drug-protein conjugate zone on the test band region. Therefore, absence of the color band on the test region indicates a positive result. [1, 2]
A control band with a different antigen/antibody reaction is added to the immune-chromatographic membrane strip at the control region (C) to indicate that the test has performed properly. This control line should always appear regardless of the presence of a drug or metabolite. If the control line does not appear, the test strip should be discarded.
The cutoff concentrations and direct calibrator for these drugs are as follows:
| Parameter | Calibrator | Cut-off (ng/mL) | Product Code |
| 7-ACL | 7-aminoclonazepam | 300 | 7-ACL-1C3-40 |
| 7-ACL | 7-aminoclonazepam | 200 | 7-ACL-1C7-40 |
| ALP | Alprazolam | 100 | ALP-1C27-40 |
| AMP | d-Amphetamine | 500 | AMP-1C9-40 |
| APVP | alpha-pyrrolidinovalerophenone | 500 | APVP-1C9-40 |
| BAR | Secobarbital | 200 | BAR-1C7-40 |
| BUP | BUP-3-D-Glucuronide | 10 | BUP-1C5-40 |
| BZO | Oxazepam | 300 | BZO-1C3-40 |
| BZO | Oxazepam | 200 | BZO-1C7-40 |
| COT | (-)-Cotinine | 1000 | COT-1C2-40 |
| HMO | Hydromorphone | 1000 | HMO-1C2-40 |
| HMO | Hydromorphone | 500 | HMO-1C9-40 |
| HMO | Hydromorphone | 250 | HMO-1C147-40 |
| K2 | JWH-073/JWH-018 | 50 | K2-1C13-40 |
| K2 | JWH-073/JWH-018 | 30 | K2-1C141-40 |
| K3 | AB-PINACA | 25 | K3-1C11-40 |
| K4 | UR-144 5-Pentanoic acid metabolite | 25 | K4-1C11-40 |
| KET | Ketamine | 1000 | KET-1C2-40 |
| KET | Ketamine | 500 | KET-1C9-40 |
| KET | Ketamine | 300 | KET-1C3-40 |
| KRA | Kratom | 500 | KRA-1C9-40 |
| LSD | Lysergic Acid Diethylamide | 50 | LSD-1C13-40 |
| MCAT | Methcathinone | 500 | MCAT-1C9-40 |
| MDA | 3,4-Methylenedioxyamphetamine | 500 | MDA-1C9-40 |
| MDMA | 3,4-Methylenedioxy​methamphetamine | 500 | MDMA-1C9-40 |
| MDPV | Methylenedioxypyrovalerone | 1000 | MDPV-1C2-40 |
| MDPV | Methylenedioxypyrovalerone | 500 | MDPV-1C9-40 |
| MEP | Mephedrone | 500 | MEP-1C9-40 |
| MES | Mescaline | 500 | MES-1C9-40 |
| MET | d-Methamphetamine | 1000 | MET-1C2-40 |
| MET | d-Methamphetamine | 500 | MET-1C9-40 |
| MET | d-Methamphetamine | 300 | MET-1C3-40 |
| MOP | Morphine | 300 | MOP-1C3-40 |
| MOP | Morphine | 200 | MOP-1C7-40 |
| MOP | Morphine | 100 | MOP-1C27-40 |
| MPD | Methylphenidate | 300 | MPD-1C3-40 |
| MPD | Methylphenidate | 150 | MPD-1C134-40 |
| MQL | Methaqualone | 300 | MQL-1C3-40 |
| MTD | Methadone | 300 | MTD-1C3-40 |
| MTD | Methadone | 200 | MTD-1C7-40 |
| NFYL | Norfentanyl | 20 | NFYL-1C48-40 |
| OPI | Opiates | 2000 | OPI-1C26-40 |
| OPI | Opiates | 1000 | OPI-1C2-40 |
| OXY | Oxycodone | 300 | OXY-1C3-40 |
| OXY | Oxycodone | 100 | OXY-1C27-40 |
| PCP | Phencyclidine | 25 | PCP-1C11-40 |
| PGB | Pregabalin | 1000 | PGB-1C2-40 |
| PGB | Pregabalin | 500 | PGB-1C9-40 |
| PPX | D-Propoxyphene | 300 | PPX-1C3-40 |
| SOMA | Carisoprodol | 1000 | SOMA-1C2-40 |
| TAP | Tapentadol | 1000 | TAP-1C2-40 |
| TCA | Nortriptyline | 1000 | TCA-1C2-40 |
| TCA | Nortriptyline | 300 | TCA-1C3-40 |
| THC | 11-nor-delta9-THC-9-COOH | 500 | THC-1C9-40 |
| THC | 11-nor-delta9-THC-9-COOH | 300 | THC-1C3-40 |
| THC | 11-nor-delta9-THC-9-COOH | 200 | THC-1C7-40 |
| THC | 11-nor-delta9-THC-9-COOH | 150 | THC-1C134-40 |
| THC | 11-nor-delta9-THC-9-COOH | 50 | THC-1C13-40 |
| THC | 11-nor-delta9-THC-9-COOH | 25 | THC-1C11-40 |
| TLD | Tilidine | 300 | TLD-1C3-40 |
| TRA | Cis-Tramadol | 300 | TRA-1C3-40 |
| TRA | Cis-Tramadol | 100 | TRA-1C27-40 |
| TPM | Tropicamide | 350 | TPM-1C151-40 |
| TZD | Trazodone | 200 | TZD-1C7-40 |
| ZAL | Zaleplon | 100 | ZAL-1C27-40 |
| ZOL | Zolpidem Phenyl-4-carboxylic | 50 | ZOL-1C13-40 |
| ZOL | Zolpidem Phenyl-4-carboxylic | 25 | ZOL-1C11-40 |
| ZOP | N-Desmethylzopiclone | 50 | ZOP-1C13-40 |
The Rapid Response™ Single Drug Test Cassette (Urine) is formulated for use with urine specimens. Fresh urine does not require any special handling or pretreatment. The urine specimen must be collected in a clean and dry container. Urine collected at any time of the day may be used. Urine specimens exhibiting visible precipitates should be centrifuged, filtered, or allowed to settle to obtain clear specimen for testing.
It is recommended the collected fresh urine to be tested immediately. Fresh urine maybe stored at room temperature (25ºC; 77°F) for up to 4 hours or to be refrigerated (2-8ºC; 36-86°F) for up to 48 hours prior to performing the test. For prolonged storage, specimens may be frozen and stored below -20ºC (-4°F). Specimens that have been refrigerated must be brought to room temperature prior to testing. Previously frozen specimens must be thawed, brought to room temperature, and mixed thoroughly prior to testing.
NOTE: Urine specimens and all materials coming in contact with them should be handled and disposed of as if capable of transmitting infection. Avoid contact with skin by wearing gloves and proper laboratory attire.
- McBay AJ. Drug-analysis technology--pitfalls and problems of drug testing. Clin Chem. 1987 Oct; 33 (11 Suppl): 33B-40B.
- Gilman AG, Goodman LS, Gilman A, eds. Goodman and Gilman's The Pharmacological Basis of Therapeutics. 6th ed. New York: Macmillan; 1980.
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